Development of a Novel Multiplex PCR Assay to Detect Functional Subtypes of KIR3DL1 Alleles
نویسندگان
چکیده
منابع مشابه
Development of a Novel Multiplex PCR Assay to Detect Functional Subtypes of KIR3DL1 Alleles
Among NK cell receptor-ligand partnerships, KIR3DL1 and HLA-Bw4 demonstrate the greatest diversity; permutations of their allelic combinations titrate NK reactivity. Balancing selection has maintained distinct subtypes of KIR3DL1 alleles in global populations, implying that each may provide unique fitness advantages and variably influence disease processes. Though approaches exist for determini...
متن کاملA multiplex real-time PCR melting curve assay to detect drug resistant mutations of Mycobacterium tuberculosis
1 Early diagnosis of drug resistant Mycobacterium tuberculosis is urgently needed to 2 optimize treatment regimens and to prevent the transmission of resistant strains. Real3 time PCR assays have been developed to detect drug resistance rapidly, but none of them 4 have been widely applied due to their complexity, high cost, or requirement for advanced 5 instruments. In this study, we developed ...
متن کاملDevelopment of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus)
A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore, this technique was found to have high specificity when t...
متن کاملdevelopment of a pcr assay to detect mycoplasma contamination in cord blood hematopoietic stem cells.
background and objectives: contamination of cell lines and biological products is one of the major problems of cell culture techniques. rapid detection of mycoplasma contamination in cell culture is an important part of quality control standards in related laboratories. the aim of this study was to evaluate the efficacy of pcr in detection of myroplasma as contaminants in cell cultures and othe...
متن کاملMultiplex real-time PCR melting curve assay to detect drug-resistant mutations of Mycobacterium tuberculosis.
Early diagnosis of drug-resistant Mycobacterium tuberculosis is urgently needed to optimize treatment regimens and to prevent the transmission of resistant strains. Real-time PCR assays have been developed to detect drug resistance rapidly, but none of them have been widely applied due to their complexity, high cost, or requirement for advanced instruments. In this study, we developed a real-ti...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: PLoS ONE
سال: 2014
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0099543